Wheat 2?Cys peroxiredoxin plays a dual role in chlorophyll biosynthesis and adaptation to high temperature

The molecular mechanism of high-temperature stress (HTS) response, in plants, has so far been investigated using transcriptomics, while the dynamics HTS-responsive proteome remain unexplored. We examined adaptive responses resilient wheat cultivar ‘Unnat Halna’ and dissected landscape. This led to identification 55 proteins (HRPs), which are predominantly involved metabolism defense pathways. Interestingly, HRPs included a 2-cysteine peroxiredoxin (2CP), designated Ta2CP, presumably perception adaptation. Complementation Ta2CP yeast heterologous expression Arabidopsis demonstrated its role thermotolerance. Both silencing overexpression inferred involvement plant growth chlorophyll biosynthesis. that interacts with protochlorophyllide reductase b, TaPORB. Reduced TaPORB was found Ta2cp-silenced upregulation observed Ta2CP-overexpressed plants. Furthermore, downregulation Taporb-silenced plants reduction suggested key metabolism. Additionally, transcript levels AGPase1 starch were increased More significantly, HTS-treated showed despite reactive oxygen species peroxide concentrations, might help rapid induction acclimation.